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The MAGUS Lum 400 fluorescent microscope is designed for diagnostic and research tasks using fluorescent (reflected light) and bright field (transmitted light) microscopic methods. With optional accessories, dark field, phase contrast and polarization techniques can also be used. Fields of application of the fluorescence microscope: DNA analysis, study of pathogens, health and epidemiological surveillance, forensic investigation, etc.
Optical elements
This is a trinocular microscope: The binocular (two-eyepiece) head part is tilted at an angle of 30°, and a digital camera (sold separately) can be installed in the three-eyepiece tube. The eyepiece tubes can be rotated 360°, so they can be easily adjusted according to the height of the user.

Five lenses can be placed in the rotating eyepiece. It comes with four infinity-plane achromatic lenses. One of them – a fluo objective with 40x magnification – was designed for fluorescence observations. 4x, 10x and 100x objectives can be used for the bright field microscopy technique. In order to achieve extra magnification within the magnification range of the microscope, an additional objective can be mounted in the free socket of the turret head.
The turret with the objectives is oriented towards the interior - the user can see the objective inserted into the optical path.

Illumination
A 100 W mercury vapor lamp serves as a reflective source. It is located inside the lamp housing, which ensures efficient heat dissipation: The lamp does not overheat even during long operating hours. The lamp can be centered along three axes, ensuring optimal illumination of the work area. If it requires replacement, removing and installing the bulb is fairly easy. The mercury vapor lamp is suitable for fluorescent dyes because it has discrete peaks. Reflector lighting contains four excitation filters: ultraviolet (UV), violet (V), blue (B), and green (G).
Transmitted light source: 30 W halogen bulb. Bright enough to use with any zoom lens. Thanks to the color temperature of the lighting, even long observations do not strain the eyes. There is also a condenser in the lighting system. There is a slot for the (phase contrast or dark field) slider.

In the case of transmitted and reflected light, the Köhler lighting method can be used. When such lighting is installed, there is no darkening at the edges, the pattern is clearly visible, and image defects are eliminated.
Stage and focusing mechanism
The microscope has an ergonomically designed stage without a positioning stand. For reflected light observation, place a black slide on the stage to eliminate stray light. The sample holder can be removed and, if necessary, removed by loosening two screws. The focus knobs are coaxial and located on both sides of the microscope. Coarse focus has a locking knob and is adjustable in tension, while fine focus is used to adjust focus.

Accessories
MAGUS Lum 400 microscope can be equipped with additional accessories – digital camera, phase contrast device, polarization device, dark field condenser, eyepieces and objectives, dark field and phase contrast slider. To measure objects, you can supplement the configuration with a calibration slide.
- Microscopic techniques: fluorescence with reflected light, clear field of view with transmitted light
- Other research methods with optional accessories: phase contrast, simple polarization, dark field of view
- Three-eye lens head part with three-eye lens tube for installing a digital camera; 360° rotatable lens tubes
- Infinity plane achromatic lenses, including a fluo lens
- Reflective light source: 100 W mercury vapor lamp, transmitted light source: 30 W halogen lamp
- Reflected and transmitted light Köhler lighting method
- Fluorescent filters: ultraviolet (UV), violet (V), blue (B), green (G)
- Ergonomic specimen table with removable sample holder, without positioning stand
Contents of the set
- Base with power input, transmitted light source and condenser, focusing mechanism, stage and turret head
- Reflective lighting
- Mercury vapor lamp
- Headpiece with three lenses
- Infinity plane achromatic objective: PL 4x/0.10 SzM 19.8 mm
- Infinity plane achromatic objective: PL 10x/0.25 SzM 5.0 mm
- Infinity plane achromatic objective, fluo: PL FL 40x/0.85 (spring) WM 0.42 mm
- Infinity plane achromatic objective: PL 100x/1.25 (spring, oil) WM 0.36 mm
- 10x/22 mm eyepiece with long eye distance (2 pcs.)
- UV shield
- C-socket adapter 1x
- Allen key
- Mercury vapor lamp power supply unit
- Power cable
- Power cable for reflective lighting
- Dust cover
- User manual and warranty card