Calibration slide for microscopes. The slide contains three horizontal micrometer scale with 100 divisions each. The scale distribution is 0.01 mm, 0.05 mm and 0.1 mm respectively. The calibration slide (also known as a concentrate micrometer) is used to determine the magnification and linear field of vision of the microscope and the distribution value of lenses and sights. With a slide and a digital camera, you can use the image analysis software to each lens.

Calibration of image analysis software
Calibration must be performed for each lens that will be used in observations. The calibration slide can be used to determine the calibration factor for the image analysis software.

Calibration procedure
- Fix the calibration slide on the microscope's table table
- Select the objective you want and maximize the resolution of the camera
- When the scale of the scale is contrastingly visible on the monitor screen, fix the image
- Choose "Calibration" mode in the image analysis software
- Click with the mouse to determine the maximum visible distance between the first and last division of the scale
- Enter the resulting scale in the image analysis software in real units
- Enter the calibration name by determining the current lens enlargement
- Check the result: In "Measurement" mode, measure some division of calibration slide scales in real units
- The image analysis software saves the calibration factor with the selected name. You will then automatically switch results to selected units

To calibrate the lens
Calibration of the lens scale must be performed for each lens, which will be used in observations. The slide should be placed on the object table and focused on the scale with the microscope. The lens should be rotated in the lens until the slide and lens scales are in parallel.

If you use small magnified lenses, you have to calculate how many divisions of the lens scale can fit into the slide scale.
When using medium and large enlarged lenses, you need to calculate how many divisions of the slide can fit into the lens scale.

The Magus CS3 calibration slide has a three micrometer scale, each with different distribution values. Calibration can be done more easily if the slide scale covers the entire lens. For small enlarged lenses, a scale of 0.1 mm or 0.05 mm is used. For lenses larger than 20x, a scale of 0.01 mm is used.

Each lens is calculated with the following formula:

- е = тl/A where:
“E is the division of the eye lens
- т The division value of the object table on the subject-to-micrometer
- l the number of divisions of the concluded tablet
А

Values ​​obtained to divide the lens scale for each lens.

When measuring an object, you first need to count how many divisions of the lens scale is covered by the object. The result must be multiplied by scale value previously calculated for the selected lens. The resulting value will be the true linear value of the object.